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Controls for e,f and g are of genotypes Pik3ca E545K, h GFAP-cre, Nestin-cre and Nestin-cre ER.
(h) MRI volumetric analyses of mutant and corresponding control brains.
The small number of cells and compact size also make it a more tractable system to investigate how complex brain structures emerge in a vertebrate organism (Pan et al. A.*, Freundlich, T., Weissman, T., Schoppik, D., Wang, C.
BAPTI and BAPTISM birthdating of neurons in zebrafish.
At E17.5, however, the number of RGCs in the VT region is similar in pigmented and albino retinae, implicating a shift in the timing of RGC production in the albino.
Short-term birthdating assays reveal a delay in RGC production in the albino VT retina between E13 and E15.
(e–g) P35 h GFAP-cre; E545K and Nestin-cre; E545K brains were noticeably larger than controls, while Nestin-cre ER; E545K mutants had normal-sized brains compared to controls.
Proper binocular vision depends on the routing at the optic chiasm of the correct proportion of retinal ganglion cell (RGC) axons that project to the same (ipsilateral) and opposite (contralateral) side of the brain.
Brd U is commonly used in the detection of proliferating cells in living tissues.
Brd U can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.
Retinal ganglion cells (RGCs) establish the connections for binocular vision by projecting their axons either to the same (ipsilateral) or the opposite (contralateral) side of the brain.
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, Brd U, BUd R, Brd Urd, broxuridine) is a synthetic nucleoside that is an analog of thymidine.
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